Analytical Data
-
Gene name
KCNK5
- Application
-
Alternative Names
KCNK5; TASK2; Potassium channel subfamily K member 5; Acid-sensitive potassium channel protein TASK-2; TWIK-related acid-sensitive K(+ channel 2
-
Species
Human
-
Source
E. coli
-
Tag
GST-tag at N-terminal
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
O95279
-
Expression Region
1-499aa
-
AA Sequence
MVDRGPLLTSAIIFYLAIGAAIFEVLEEPHWKEAKKNYYTQKLHLLKEFPCLGQEGLDKILEVVSDAAGQGVAITGNQTFNNWNWPNAMIFAATVITTIGYGNVAPKTPAGRLFCVFYGLFGVPLCLTWISALGKFFGGRAKRLGQFLTKRGVSLRKAQITCTVIFIVWGVLVHLVIPPFVFMVTEGWNYIEGLYYSFITISTIGFGDFVAGVNPSANYHALYRYFVELWIYLGLAWLSLFVNWKVSMFVEVHKAIKKRRRRRKESFESSPHSRKALQVKGSTASKDVNIFSFLSKKEETYNDLIKQIGKKAMKTSGGGETGPGPGLGPQGGGLPALPPSLVPLVVYSKNRVPTLEEVSQTLRSKGHVSRSPDEEAVARAPEDSSPAPEVFMNQLDRISEECEPWDAQDYHPLIFQDASITFVNTEAGLSDEETSKSSLEDNLAGEESPQQGAEAKAPLNMGEFPSSSESTFTSTESELSVPYEQLMNEYNKANSPKGT
-
Molecular Weight
80.63 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
KCNK5, also known as TWIK-2, is a member of the two-pore domain potassium channel family, which plays a critical role in maintaining the resting membrane potential and regulating excitability in various cell types. The study of KCNK5 is particularly significant due to its involvement in numerous physiological processes and its potential implications in pathophysiological conditions, including hypertension, pain sensation, and certain neurodegenerative diseases. Recent research has highlighted its distinct biophysical properties and pharmacological profiles, making it a potential target for therapeutic interventions. Recombinant KCNK5 protein has been increasingly utilized in studies aiming to elucidate its structure-function relationships, ion conductance mechanisms, and modulation by lipids and pharmacological agents. Furthermore, understanding KCNK5’s role in cellular signaling pathways can provide insights into how alterations in its function contribute to disease states. This research not only enhances our knowledge of potassium channel biology but also paves the way for the development of novel strategies for drug design targeting potassium channels. Overall, the exploration of KCNK5 recombinant protein serves as a crucial step in advancing both fundamental and applied aspects of ion channel research.
