Analytical Data
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Gene name
KCNK1
- Application
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Alternative Names
KCNK1; HOHO1; KCNO1; TWIK1; Potassium channel subfamily K member 1; Inward rectifying potassium channel protein TWIK-1; Potassium channel K2P1; Potassium channel KCNO1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O00180
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Expression Region
1-336aa
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AA Sequence
MLQSLAGSSCVRLVERHRSAWCFGFLVLGYLLYLVFGAVVFSSVELPYEDLLRQELRKLKRRFLEEHECLSEQQLEQFLGRVLEASNYGVSVLSNASGNWNWDFTSALFFASTVLSTTGYGHTVPLSDGGKAFCIIYSVIGIPFTLLFLTAVVQRITVHVTRRPVLYFHIRWGFSKQVVAIVHAVLLGFVTVSCFFFIPAAVFSVLEDDWNFLESFYFCFISLSTIGLGDYVPGEGYNQKFRELYKIGITCYLLLGLIAMLVVLETFCELHELKKFRKMFYVKKDKDEDQVHIIEHDQLSFSSITDQAAGMKEDQKQNEPFVATQSSACVDGPANH
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Molecular Weight
38.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
KCNK1, also known as TASK-1, is a member of the two-pore domain potassium (K2P) channel family, which plays a crucial role in maintaining the resting membrane potential and regulating cellular excitability in various tissues. The investigation of KCNK1 recombinant protein is significant due to its involvement in physiological processes such as neuronal excitability, cardiovascular function, and respiratory control. Dysregulation or altered expression of KCNK1 has been associated with various pathophysiological conditions, including mood disorders, sleep disturbances, and certain types of cancer. The study of KCNK1 in a recombinant form allows researchers to explore its functional properties, ion selectivity, and gating mechanisms in a controlled environment, free from the complexities of cellular systems. Furthermore, characterizing KCNK1's pharmacological profile can aid in the development of targeted therapeutics, potentially leading to novel treatment strategies for diseases linked to potassium channel dysfunction. By utilizing techniques such as heterologous expression in systems like HEK293 or oocytes, researchers can analyze the biophysical attributes of KCNK1, including its response to various modulators and its interaction with signaling pathways. Thus, KCNK1 recombinant protein research not only contributes to the fundamental understanding of ion channel biology but also holds promise for advancing clinical applications in neurology, cardiology, and oncology.
