Analytical Data
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Gene name
P4Ha3
- Application
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Alternative Names
P4Ha3；Prolyl 4-hydroxylase subunit alpha-3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q7Z4N8
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Expression Region
20-544aa
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AA Sequence
DPERAAARGDTFSALTSVARALAPERRLLGLLRRYLRGEEARLRDLTRFYDKVLSLHEDSTTPVANPLLAFTLIKRLQSDWRNVVHSLEASENIRALKDGYEKVEQDLPAFEDLEGAARALMRLQDVYMLNVKGLARGVFQRVTGSAITDLYSPKRLFSLTGDDCFQVGKVAYDMGDYYHAIPWLEEAVSLFRGSYGEWKTEDEASLEDALDHLAFAYFRAGNVSCALSLSREFLLYSPDNKRMARNVLKYERLLAESPNHVVAEAVIQRPNIPHLQTRDTYEGLCQTLGSQPTLYQIPSLYCSYETNSNAYLLLQPIRKEVIHLEPYIALYHDFVSDSEAQKIRELAEPWLQRSVVASGEKQLQVEYRISKSAWLKDTVDPKLVTLNHRIAALTGLDVRPPYAEYLQVVNYGIGGHYEPHFDHATSPSSPLYRMKSGNRVATFMIYLSSVEAGGATAFIYANLSVPVVRNAALFWWNLHRSGEGDSDTLHAGCPVLVGDKWVANKWIHEYGQEFRRPCSSSPED
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Molecular Weight
75.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
P4Ha3, a member of the prolyl 4-hydroxylase family, plays a crucial role in collagen biosynthesis by catalyzing the hydroxylation of proline residues in collagen precursors, which is essential for the stability and functionality of collagen fibers. Alterations in the activity of P4Ha3 have been linked to various pathological conditions, such as fibrosis, cancer, and cardiovascular diseases, highlighting its significance in modulating extracellular matrix (ECM) dynamics. Research on P4Ha3 has focused on understanding its biochemical properties, regulatory mechanisms, and interactions with other proteins involved in collagen formation. Some studies have explored the therapeutic potential of targeting P4Ha3 to enhance collagen production or to mitigate fibrotic diseases. Additionally, the expression levels of P4Ha3 can serve as biomarkers for certain diseases, making it a promising candidate for diagnostic applications. Given the rising interest in the development of antifibrotic therapies, the characterization of P4Ha3 and its role in ECM remodeling is of paramount importance for advancing our understanding of tissue homeostasis and pathology.
