Analytical Data
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Gene name
TRPM1
- Application
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Alternative Names
TRPM1；LTRPC1；MLSN；MLSN1；Transient receptor potential cation channel subfamily M member 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q7Z4N2
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Expression Region
1127-1603aa
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AA Sequence
RCRKKREGDQEERDRGLKLFLSDEELKRLHEFEEQCVQEHFREKEDEQQSSSDERIRVTSERVENMSMRLEEINERETFMKTSLQTVDLRLAQLEELSNRMVNALENLAGIDRSDLIQARSRASSECEATYLLRQSSINSADGYSLYRYHFNGEELLFEDTSLSTSPGTGVRKKTCSFRIKEEKDVKTHLVPECQNSLHLSLGTSTSATPDGSHLAVDDLKNAEESKLGPDIGISKEDDERQTDSKKEETISPSLNKTDVIHGQDKSDVQNTQLTVETTNIEGTISYPLEETKITRYFPDETINACKTMKSRSFVYSRGRKLVGGVNQDVEYSSITDQQLTTEWQCQVQKITRSHSTDIPYIVSEAAVQAEHKEQFADMQDEHHVAEAIPRIPRLSLTITDRNGMENLLSVKPDQTLGFPSLRSKSLHGHPRNVKSIQGKLDRSGHASSVSSLVIVSGMTAEEKKVKKEKASTETEC
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Molecular Weight
61.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
TRPM1 (Transient Receptor Potential Melastatin 1) is a member of the TRP ion channel family, which plays a crucial role in sensory transduction, particularly in the visual system. It is primarily expressed in retinal ON-bipolar cells and has been implicated in the phototransduction pathway, where it contributes to the regulation of intracellular calcium levels. Mutations in the TRPM1 gene are associated with various forms of retinal degeneration, resulting in night blindness and vision loss. Given its significance in vision, TRPM1 has garnered attention for its potential therapeutic implications in retinal diseases. The study of TRPM1 recombinant proteins is critical for understanding its structure-function relationships and developing pharmacological agents that could modulate its activity. By expressing and purifying TRPM1 as a recombinant protein, researchers aim to investigate its biophysical properties, ligand interactions, and the mechanisms underlying its ion channel activity. Such studies could provide insights into the pathophysiology of retinal disorders and open avenues for innovative treatments targeting TRPM1 dysfunction. As research advances, elucidating the precise role of TRPM1 in both healthy and diseased states could lead to breakthroughs in restoring vision and enhancing our understanding of sensory processing at the cellular level.
