Analytical Data
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Gene name
Mettl10
- Application
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Alternative Names
Mettl10；C10orf138；METTL10；EEF1A lysine methyltransferase 2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q5JPI9
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Expression Region
2-291aa
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AA Sequence
SSGADGGGG AAVAARSDKG SPGEDGFVPS ALGTREHWDA VYERELQTFR EYGDTGEIWF GEESMNRLIR WMQKHKIPLD ASVLDIGTGN GVFLVELAKF GFSNITGIDY SPSAIQLSGS IIEKEGLSNI KLKVEDFLNL STQLSGFHIC IDKGTFDAIS LNPDNAIEKR KQYVKSLSRV LKVKGFFLIT SCNWTKEELL NEFSEGWSTV AGFWLTAALT SWAQAIFSTS ASRVGGTTGT HHHAWIIFVF LAETRFCHVV QAGLELLGSS DSPTWPPKVL GLYHARPSLA F
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Molecular Weight
25.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Mettl10, a member of the METTL family of methyltransferases, has gained attention in recent years due to its potential role in RNA methylation and post-transcriptional regulation. Research indicates that Mettl10 may be involved in mediating various biological processes, such as cell differentiation, proliferation, and stress response, by modifying RNA molecules through the addition of methyl groups. This modification can influence RNA stability, splicing, and translation efficiency, thereby impacting gene expression levels. Given that dysregulation of RNA methylation has been linked to various diseases, including cancer, understanding the functional role of Mettl10 could provide insights into its molecular mechanisms and therapeutic potential. Recent studies utilizing recombinant Mettl10 proteins have enabled researchers to dissect the enzymatic activity and substrate specificity of this methyltransferase, paving the way for the development of novel strategies to target RNA modifications in disease contexts. The exploration of Mettl10's structure-function relationship and its interactions with RNA and other cellular factors is essential for unraveling its contribution to cellular homeostasis and disease pathology.
