Analytical Data
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Gene name
HUNK
- Application
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Alternative Names
B19; Bstk1; Hormonally up-regulated neu tumor-associated kinase; Hormonally upregulated neu associated kinase; Hormonally upregulated neu tumor associated kinase; HUNK; HUNK_HUMAN; MAKV; Serine/threonine-protein kinase MAK-V
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P57058
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Expression Region
1-714aa
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AA Sequence
MPAAAGDGLL GEPAAPGGGG GAEDAARPAA ACEGSFLPAW VSGVPRERLR DFQHHKRVGN YLIGSRKLGE GSFAKVREGL HVLTGEKVAI KVIDKKRAKK DTYVTKNLRR EGQIQQMIRH PNITQLLDIL ETENSYYLVM ELCPGGNLMH KIYEKKRLEE SEARRYIRQL ISAVEHLHRA GVVHRDLKIE NLLLDEDNNI KLIDFGLSNC AGILGYSDPF STQCGSPAYA APELLARKKY GPKIDVWSIG VNMYAMLTGT LPFTVEPFSL RALYQKMVDK EMNPLPTQLS TGAISFLRSL LEPDPVKRPN IQQALANRWL NENYTGKVPC NVTYPNRISL EDLSPSVVLH MTEKLGYKNS DVINTVLSNR ACHILAIYFL LNKKLERYLS GKSDIQDSLC YKTRLYQIEK YRAPKESYEA SLDTWTRDLE FHAVQDKKPK EQEKRGDFLH RPFSKKLDKN LPSHKQPSGS LMTQIQNTKA LLKDRKASKS SFPDKDSFGC RNIFRKTSDS NCVASSSMEF IPVPPPRTPR IVKKPEPHQP GPGSTGIPHK EDPLMLDMVR SFESVDRDDH VEVLSPSHHY RILNSPVSLA RRNSSERTLS PGLPSGSMSP LHTPLHPTLV SFAHEDKNSP PKEEGLCCPP PVPSNGPMQP LGSPNCVKSR GRFPMMGIGQ MLRKRHQSLQ PSADRPLEAS LPPLQPLAPV NLAFDMADGV KTQC
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Molecular Weight
79.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
HUNK (Hormonally Upregulated Neu tumor kinase) is a serine/threonine kinase that has gained attention in cancer research due to its differential expression in various tumors and its involvement in cell proliferation, apoptosis, and tumor progression. Initially identified as an estrogen-responsive gene, HUNK plays a significant role in the signaling pathways that regulate cellular responses to hormonal stimuli, making it a potential target for cancer therapies. Studies have indicated that HUNK is overexpressed in several malignancies, including breast and prostate cancers, suggesting a correlation with aggressive tumor behavior and poor prognosis. Furthermore, HUNK has been implicated in the promotion of cellular motility and metastasis, highlighting its potential role in cancer metastasis. Given its diverse biological functions and involvement in key oncogenic processes, researchers are increasingly focused on understanding the mechanisms by which HUNK contributes to tumorigenesis. The development of recombinant HUNK proteins can facilitate elucidation of its structure-function relationships, functional assays, and interaction studies with other signaling molecules. This research is crucial for unraveling HUNK's role in cancer and developing targeted therapeutic strategies. Understanding HUNK's signaling mechanisms may provide insights into novel treatment options for cancers characterized by its overexpression, thereby advancing personalized medicine approaches in oncology.
