Analytical Data
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Gene name
HDAC8
- Application
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Alternative Names
HDAC8;HDACL1;Histone deacetylase 8
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BY41
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Expression Region
1-377aa
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AA Sequence
MEEPEEPADS GQSLVPVYIY SPEYVSMCDS LAKIPKRASM VHSLIEAYAL HKQMRIVKPK VASMEEMATF HTDAYLQHLQ KVSQEGDDDH PDSIEYGLGY DCPATEGIFD YAAAIGGATI TAAQCLIDGM CKVAINWSGG WHHAKKDEAS GFCYLNDAVL GILRLRRKFE RILYVDLDLH HGDGVEDAFS FTSKVMTVSL HKFSPGFFPG TGDVSDVGLG KGRYYSVNVP IQDGIQDEKY YQICESVLKE VYQAFNPKAV VLQLGADTIA GDPMCSFNMT PVGIGKCLKY ILQWQLATLI LGGGGYNLAN TARCWTYLTG VILGKTLSSE IPDHEFFTAY GPDYVLEITP SCRPDRNEPH RIQQILNYIK GNLKHVV
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Histone deacetylase 8 (HDAC8) is an important enzyme that belongs to the class I HDAC family, playing a pivotal role in the regulation of various cellular processes, including gene expression, differentiation, and apoptosis. The dysregulation of HDAC8 has been implicated in several diseases, particularly cancer, making it a key target for therapeutic interventions. HDAC8 catalyzes the removal of acetyl groups from lysine residues on histone and non-histone proteins, leading to a more compact and transcriptionally inactive chromatin structure. Recent research has shown that HDAC8 is involved in multiple signaling pathways and cellular functions beyond chromatin remodeling, such as the regulation of protein stability and interaction. Consequently, understanding the structure and function of HDAC8 is crucial for developing selective inhibitors and novel therapeutic strategies. The production of recombinant HDAC8 protein allows researchers to investigate its biochemical properties, enzyme kinetics, and interactome in detail. By utilizing techniques such as X-ray crystallography and NMR spectroscopy, scientists aim to elucidate the molecular mechanisms by which HDAC8 exerts its effects, paving the way for the design of drugs that could selectively modulate its activity in pathological conditions. As ongoing studies continue to explore HDAC8's role in health and disease, the demand for recombinant HDAC8 protein is expected to grow, offering promising avenues for targeted therapy in various malignancies and other HDAC8-related disorders.
