Analytical Data
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Gene name
AMY1A
- Application
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Alternative Names
AMY1A；AMY1；Alpha-amylase 1A
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P04745
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Expression Region
16-511aa
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AA Sequence
QYSSN TQQGRTSIVH LFEWRWVDIA LECERYLAPK GFGGVQVSPP NENVAIHNPF RPWWERYQPV SYKLCTRSGN EDEFRNMVTR CNNVGVRIYV DAVINHMCGN AVSAGTSSTC GSYFNPGSRD FPAVPYSGWD FNDGKCKTGS GDIENYNDAT QVRDCRLSGL LDLALGKDYV RSKIAEYMNH LIDIGVAGFR IDASKHMWPG DIKAILDKLH NLNSNWFPEG SKPFIYQEVI DLGGEPIKSS DYFGNGRVTE FKYGAKLGTV IRKWNGEKMS YLKNWGEGWG FMPSDRALVF VDNHDNQRGH GAGGASILTF WDARLYKMAV GFMLAHPYGF TRVMSSYRWP RYFENGKDVN DWVGPPNDNG VTKEVTINPD TTCGNDWVCE HRWRQIRNMV NFRNVVDGQP FTNWYDNGSN QVAFGRGNRG FIVFNNDDWT FSLTLQTGLP AGTYCDVISG DKINGNCTGI KIYVSDDGKA HFSISNSAED PFIAIHAESK L
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The AMY1A gene encodes the enzyme salivary amylase, which plays a crucial role in the initial digestion of carbohydrates in the human body. Variability in the copy number of the AMY1A gene among different populations has drawn significant attention in recent years, as it appears to correlate with dietary habits and starch consumption. Individuals from populations with high-starch diets tend to have more copies of the AMY1A gene, suggesting an adaptive evolutionary response that enhances their ability to digest starches efficiently. The study of recombinant AMY1A protein is pivotal for understanding its structural and functional properties, aiding in elucidating the molecular mechanisms behind starch digestion. By producing and characterizing the recombinant protein, researchers can investigate its enzymatic activity, stability, and potential interactions with other biomolecules. This research not only contributes to a fundamental understanding of human digestive physiology but may also have implications for developing dietary interventions and therapeutic strategies for metabolic disorders linked to carbohydrate intake. Understanding the variability and function of AMY1A can provide insights into the evolution of human diets and their impacts on health, making it a significant focus in both nutritional science and evolutionary biology.
