Analytical Data
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基因名
IFN-gamma R1/CD119
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简介
IFN-gamma R1 (CD119), one of the subunit of IFN-gamma receptor, is a receptor for IFN-gamma. IFN-gamma R1 forms the functional receptor with IFN-gamma R2. Upon binding with IFN-gamma,IFN-gamma R1 and IFN-gamma R2 oligomerize and transphosphorylate[1]. Then, JAK1 and JAK2 are phosphorylated and activated, and STAT1 is recruited to the receptor complex. Phosphorylated STAT1 translocates to the nucleus, where it regulates the expression of IFN-responsive genes (e.g. CD54)[2]. IFN-gamma R1/CD119 Protein, Human (HEK293, His, solution) is a recombinant human IFN-gamma R1 (E18-G245) with C-terminal 6*His tag, which is produced in HEK293 cells.
- Application
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别名
Interferon gamma receptor 1; IFN-gamma-R1; IFN-gamma-R-alpha; CD119; Ifngr1
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种属
Human
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P15260-1
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表达区间
E18-G245
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氨基酸序列
EMGTADLGPSSVPTPTNVTIESYNMNPIVYWEYQIMPQVPVFTVEVKNYGVKNSEWIDACINISHHYCNISDHVGDPSNSLWVRVKARVGQKESAYAKSEEFAVCRDGKIGPPKLDIRKEEKQIMIDIFHPSVFVNGDEQEVDYDPETTCYIRVYNVYVRMNGSEIQYKILTQKEDDCDEIQCQLAIPVSSLNSQYCVSAEGVLHVWGVTTEKSKEVCITIFNSSIKG
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蛋白长度
Extracellular Domain
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分子量
40-50 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
IFN-gamma receptor 1 (IFN-gamma R1 or CD119) plays a crucial role in the immune response by mediating the effects of interferon-gamma (IFN-γ), a key cytokine involved in the regulation of immune functions, particularly in activating macrophages and promoting Th1 responses. The binding of IFN-γ to its receptor induces a signaling cascade that activates several pathways leading to the expression of genes important for pathogen clearance and immune regulation. Due to its significant role in immunity, CD119 is a critical target for research in various fields, including infectious diseases, cancer therapy, and autoimmune disorders. Recombinant proteins of IFN-gamma R1 are produced for various purposes, including elucidating the receptor's structural characteristics, understanding its signaling mechanisms, and developing therapeutic strategies. Studies involving the recombinant form help investigate the receptor's interaction with its ligand and other proteins, enabling the identification of novel therapeutic targets. Additionally, the recombinant protein can be used in vaccine development and diagnostic assays, enhancing our understanding of immune responses in health and disease. Overall, the research surrounding IFN-gamma R1/CD119 recombinant protein is vital for advancing our knowledge of immune regulation and developing targeted treatments to modulate the immune system effectively.
