Analytical Data
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基因名
HTRA4
- Application
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别名
HTRA4;Serine protease HTRA4
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P83105
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表达区间
159-376aa
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氨基酸序列
SAGPLRRNYNFIAAVVEKVAPSVVHVQLWGRLLHGSRLVPVYSGSGFIVS EDGLIITNAHVVRNQQWIEVVLQNGARYEAVVKDIDLKLDLAVIKIESNA ELPVLMLGRSSDLRAGEFVVALGSPFSLQNTATAGIVSTKQRGGKELGMK DSDMDYVQIDATINYGNSGGPLVNLDGDVIGVNSLRVTDGISFAIPSDRV RQFLAEYHEHQMKGKAFS
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分子量
25 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HTRA4, a member of the high-temperature requirement A (HTRA) serine protease family, has garnered significant attention in recent years due to its multifaceted roles in various biological processes and its potential implications in human diseases. Initially characterized for its involvement in extracellular matrix remodeling and proteolytic activity, HTRA4 has emerged as a critical player in both normal physiological functions and pathological conditions, including cancer and cardiovascular diseases. Research has shown that HTRA4 regulates key signaling pathways and influences cellular processes such as apoptosis, proliferation, and inflammation. Furthermore, atypical expression levels of HTRA4 have been implicated in tumor progression and metastasis, making it a potential biomarker for cancer diagnostics and a target for therapeutic intervention. The recombinant expression of HTRA4 provides a valuable tool for elucidating its specific functions and mechanisms of action. As the understanding of HTRA4's role in health and disease continues to deepen, the development of recombinant HTRA4 protein is essential for conducting detailed studies, including its interactions with substrates and inhibitors. This research could pave the way for novel therapeutic strategies aimed at modulating HTRA4 activity in various pathological contexts, ultimately contributing to improved disease management and patient outcomes.












