Analytical Data
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Gene name
HAL
- Application
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Alternative Names
IS; hutH 1; Hal; HAL protein; Histidase; Histidine ammonia lyase; Histidine ammonia-lyase; HSTD; HutH; HUTH_HUMAN
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P42357
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Expression Region
1-657aa
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AA Sequence
MPRYTVHVRGEWLAVPCQDAQLTVGWLGREAVRRYIKNKPDNGGFTSVDDAHFLVRRCKGLGLLDNEDRLEVALENNEFVEVVIEGDAMSPDFIPSQPEGVYLYSKYREPEKYIELDGDRLTTEDLVNLGKGRYKIKLTPTAEKRVQKSREVIDSIIKEKTVVYGITTGFGKFARTVIPINKLQELQVNLVRSHSSGVGKPLSPERCRMLLALRINVLAKGYSGISLETLKQVIEMFNASCLPYVPEKGTVGASGDLAPLSHLALGLVGEGKMWSPKSGWADAKYVLEAHGLKPVILKPKEGLALINGTQMITSLGCEAVERASAIARQADIVAALTLEVLKGTTKAFDTDIHALRPHRGQIEVAFRFRSLLDSDHHPSEIAESHRFCDRVQDAYTLRCCPQVHGVVNDTIAFVKNIITTELNSATDNPMVFANRGETISGGNFHGEYPAKALDYLAIGIHELAAISERRIERLCNPSLSELPAFLVAEGGLNSGFMIAHCTAAALVSENKALCHPSSVDSLSTSAATEDHVSMGGWAARKALRVIEHVEQVLAIELLAACQGIEFLRPLKTTTPLEKVYDLVRSVVRPWIKDRFMAPDIEAAHRLLLEQKVWEVAAPYIEKYRMEHIPESRPLSPTAFSLQFLHKKSTKIPESEDL
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Molecular Weight
99.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
HAL (Histamine N-Methyltransferase) is an enzyme involved in the metabolism of histamine, a biogenic amine that plays crucial roles in various physiological processes, including immune response, gastric acid secretion, and neurotransmission. The research on HAL recombined proteins has gained prominence due to their potential therapeutic applications in managing allergic reactions, neurological disorders, and gastric diseases. By understanding the structure and function of HAL, researchers aim to develop novel interventions that can modulate histamine levels in the body. Recombinant HAL proteins can be produced using advanced biotechnological methods, allowing for the study of enzyme kinetics, substrate specificity, and the development of inhibitors or activators. Furthermore, as histamine-related disorders become increasingly prevalent in modern society, the demand for efficient treatments underscores the importance of HAL in biomedical research. Investigating HAL’s role in disease mechanisms also opens new avenues for drug discovery and personalized medicine, making it a critical target for therapeutic exploration. Thus, the study of HAL recombined proteins not only enhances our understanding of histamine metabolism but also holds promise for clinical advancements in the treatment of several histamine-related health issues.
