Analytical Data
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Gene name
GRSF1
- Application
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Alternative Names
G rich RNA sequence binding factor 1; G rich sequence binding factor 1; G-rich sequence factor 1; GRSF-1; GRSF1; GRSF1_HUMAN
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q12849
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Expression Region
1-459aa
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AA Sequence
MAGTRWVLGALLRGCGCNCSSCRRTGAACLPFYSAAAAASQTRGLQTGPVPPGRLAGPPAVATSAAAAAAASYPALRASLLPQSLAAAAAVPTRSYSQESKTTYLEDLPPPPEYELAPSKLEEEVDDVFLIRAQGLPWSCTMEDVLNFFSDCRIRNGENGIHFLLNRDGKRRGDALIEMESEQDVQKALEKHRMYMGQRYVEVYEINNEDVDALMKSLQVKSSPVVNDGVVRLRGLPYSCNEKDIVDFFAGLNIVDITFVMDYRGRRKTGEAYVQFEEPEMANQALLKHREEIGNRYIEIFPSRRNEVRTHVGSYKGKKIASFPTAKYITEPEMVFEEHEVNEDIQPMTAFESEKEIELPKEVPEKLPEAADFGTTSSLHFVHMRGLPFQANAQDIINFFAPLKPVRITMEYSSSGKATGEADVHFETHEDAVAAMLKDRSHVHHRYIELFLNSCPKGK
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Molecular Weight
77.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GRSF1 (G-rich sequence binding factor 1) is a member of the RNA-binding protein family that has garnered attention for its role in post-transcriptional regulation of gene expression. Emerging studies indicate that GRSF1 is involved in various biological processes, including cellular stress response, apoptosis, and regulation of translation, particularly in the context of cancer and neurodegenerative diseases. The protein possesses RNA recognition motifs that allow it to bind to specific RNA sequences, suggesting its potential involvement in modulating mRNA stability and translation efficiency. The significance of GRSF1 is further underscored by its differential expression in various tissues and its association with critical pathways that could influence tumorigenesis and neuronal function. Despite these insights, detailed mechanistic understanding of GRSF1's actions at the molecular level remains limited, driving the need for comprehensive studies including the characterization of recombinant GRSF1 proteins. The recombinant production of GRSF1 provides a valuable tool to explore its biochemical properties, interaction partners, and functional roles in cellular contexts. Through structural and functional analyses of the recombinant protein, researchers aim to elucidate the precise mechanisms by which GRSF1 exerts its regulatory effects, thereby paving the way for potential therapeutic applications in diseases where GRSF1 is implicated.
