Analytical Data
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Gene name
IFN-beta
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简介
IFN-beta 是一种分泌性细胞因子，由细胞在病毒感染或核酸刺激下产生。IFN-beta 与细胞表面异二聚体受体 (IFNAR1/IFNAR2) 结合，激活 JAK-STAT 信号通路，诱导干扰素刺激基因 (ISG) (如 PKR、2'5'-OAS) 的表达。IFN-beta 能够抑制病毒增殖，调节免疫反应 (如抑制Th1细胞极化、减少促炎细胞因子分泌)，抑制血管平滑肌细胞和肿瘤细胞的增殖。IFN-beta 具有抗动脉粥样硬化和血管重塑作用。IFN-beta Protein, Human (CHO) 是由 CHO 表达的重组干扰素 β 蛋白，无标签。
- Application
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Biological Activity
1.Measured in antiviral assays using WISH cells infected with vesicular stomatitis virus and the ED50 is 1-20 pg/mL. 2.Determined by a cytotoxicity assay using human TF-1 cells. The ED50 for this effect is <0>1 x 107 units/mg. Determined by a cytotoxicity assay using human TF-1 cells. The ED50 for this effect is 0.07167 ng/mL,corresponding to a specific activity of 1.395 x 107 units/mg.
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Alternative Names
Interferon beta; IFN-beta; IFNB1; IFB; IFNB
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Species
Human
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Source
CHO
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Tag
Tag Free
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
P01574
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Expression Region
M22-N187
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Protein Length
Full Length of Mature Protein
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Molecular Weight
20-23 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Interferon-beta (IFN-β) is a crucial cytokine with significant roles in the immune response, particularly in antiviral defense and modulation of the immune system. Its therapeutic potential has garnered substantial attention, especially in the context of multiple sclerosis (MS) treatment, where it is utilized to reduce the frequency of relapses and slow disease progression. The recombinant production of IFN-β using recombinant DNA technology has enabled the generation of high-purity and biologically active proteins, initially derived from human cells. Research on IFN-β has expanded to explore its mechanisms of action, including the activation of immune cells, the inhibition of viral replication, and its impact on the central nervous system. Furthermore, studies have focused on optimizing its formulation for enhanced stability and efficacy. Advances in biotechnological methods, such as improved expression systems and purification techniques, have led to the development of various pharmaceutical formulations of IFN-β, making it a cornerstone in the treatment landscape of autoimmune and viral diseases. Ongoing research continues to investigate its application in other conditions, such as cancers and other inflammatory disorders, reinforcing its position as a critical component in the therapeutic arsenal against various pathologies.
