Analytical Data
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Gene name
IFN-beta
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简介
IFN-beta is a secretory cytokine produced by cells under viral infection or nucleic acid stimulation. IFNβ binds to cell surface heterodimeric receptors (IFNAR1/IFNAR2), activates the JAK-STAT signaling pathway, and induces the expression of interferon-stimulated genes (ISGs) (such as PKR, 2'5'-OAS). IFN-beta inhibits viral proliferation, regulates immune responses (such as inhibiting Th1 cell polarization, reducing pro-inflammatory cytokine secretion), inhibits the proliferation of vascular smooth muscle cells and tumor cells. IFN-beta has anti-atherosclerotic and vascular remodeling effects. Mouse (HEK293) is the recombinant mouse-derived IFN-beta protein, expressed by HEK293 , with tag free.
- Application
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Biological Activity
1. Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV) and the ED50 is 3-18 pg/mL. 2. Determined by a cytotoxicity assay using human TF-1 cells. The ED50 this effect is ≤0.3901 ng/mL, corresponding to a specific activity is ≥2.563×106 units/mg. 3. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse IFNAR2 at 2 μg/mL (100 μL/well) can bind Biotinylated Recombinant Mouse IFN-beta. The ED50 for this effect is <5 ng/mL. Determined by a cytotoxicity assay using human TF-1 cells. The ED50 for this effect is 0.3901 ng/mL, corresponding to a specific activity is 2.563×106 units/mg.
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Alternative Names
Interferon beta; IFN-beta; IFNB1; IFB; IFNB
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Species
Mouse
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Source
HEK293
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P01575
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Expression Region
I22-N182
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Protein Length
Full Length of Mature Protein
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Molecular Weight
24-33 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Interferon-beta (IFN-β) is a crucial cytokine with significant roles in the immune response, particularly in antiviral defense and modulation of the immune system. Its therapeutic potential has garnered substantial attention, especially in the context of multiple sclerosis (MS) treatment, where it is utilized to reduce the frequency of relapses and slow disease progression. The recombinant production of IFN-β using recombinant DNA technology has enabled the generation of high-purity and biologically active proteins, initially derived from human cells. Research on IFN-β has expanded to explore its mechanisms of action, including the activation of immune cells, the inhibition of viral replication, and its impact on the central nervous system. Furthermore, studies have focused on optimizing its formulation for enhanced stability and efficacy. Advances in biotechnological methods, such as improved expression systems and purification techniques, have led to the development of various pharmaceutical formulations of IFN-β, making it a cornerstone in the treatment landscape of autoimmune and viral diseases. Ongoing research continues to investigate its application in other conditions, such as cancers and other inflammatory disorders, reinforcing its position as a critical component in the therapeutic arsenal against various pathologies.
