Analytical Data
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基因名
IFN-beta
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简介
IFN-beta is a secretory cytokine produced by cells under viral infection or nucleic acid stimulation. IFNβ binds to cell surface heterodimeric receptors (IFNAR1/IFNAR2), activates the JAK-STAT signaling pathway, and induces the expression of interferon-stimulated genes (ISGs) (such as PKR, 2'5'-OAS). IFN-beta inhibits viral proliferation, regulates immune responses (such as inhibiting Th1 cell polarization, reducing pro-inflammatory cytokine secretion), inhibits the proliferation of vascular smooth muscle cells and tumor cells. IFN-beta has anti-atherosclerotic and vascular remodeling effects. Mouse (HEK293) is the recombinant mouse-derived IFN-beta protein, expressed by HEK293 , with tag free.
- Application
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生物活性
1. Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV) and the ED50 is 3-18 pg/mL. 2. Determined by a cytotoxicity assay using human TF-1 cells. The ED50 this effect is ≤0.3901 ng/mL, corresponding to a specific activity is ≥2.563×106 units/mg. 3. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse IFNAR2 at 2 μg/mL (100 μL/well) can bind Biotinylated Recombinant Mouse IFN-beta. The ED50 for this effect is <5 ng/mL. Determined by a cytotoxicity assay using human TF-1 cells. The ED50 for this effect is 0.3901 ng/mL, corresponding to a specific activity is 2.563×106 units/mg.
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别名
Interferon beta; IFN-beta; IFNB1; IFB; IFNB
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种属
Mouse
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表达系统
HEK293
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标签
Tag Free
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P01575
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表达区间
I22-N182
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蛋白长度
Full Length of Mature Protein
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分子量
24-33 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Interferon-beta (IFN-β) is a crucial cytokine with significant roles in the immune response, particularly in antiviral defense and modulation of the immune system. Its therapeutic potential has garnered substantial attention, especially in the context of multiple sclerosis (MS) treatment, where it is utilized to reduce the frequency of relapses and slow disease progression. The recombinant production of IFN-β using recombinant DNA technology has enabled the generation of high-purity and biologically active proteins, initially derived from human cells. Research on IFN-β has expanded to explore its mechanisms of action, including the activation of immune cells, the inhibition of viral replication, and its impact on the central nervous system. Furthermore, studies have focused on optimizing its formulation for enhanced stability and efficacy. Advances in biotechnological methods, such as improved expression systems and purification techniques, have led to the development of various pharmaceutical formulations of IFN-β, making it a cornerstone in the treatment landscape of autoimmune and viral diseases. Ongoing research continues to investigate its application in other conditions, such as cancers and other inflammatory disorders, reinforcing its position as a critical component in the therapeutic arsenal against various pathologies.
