Analytical Data
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Gene name
KCC4
- Application
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Alternative Names
KCC4；CAMK；CAMK-GR；CAMKIV；Calcium/calmodulin-dependent Protein kinase type IV
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q16566
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Expression Region
1-473aa
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AA Sequence
MLKVTVPSCSASSCSSVTASAAPGTASLVPDYWIDGSNRDALSDFFEVESELGRGATSIVYRCKQKGTQKPYALKVLKKTVDKKIVRTEIGVLLRLSHPNIIKLKEIFETPTEISLVLELVTGGELFDRIVEKGYYSERDAADAVKQILEAVAYLHENGIVHRDLKPENLLYATPAPDAPLKIADFGLSKIVEHQVLMKTVCGTPGYCAPEILRGCAYGPEVDMWSVGIITYILLCGFEPFYDERGDQFMFRRILNCEYYFISPWWDEVSLNAKDLVRKLIVLDPKKRLTTFQALQHPWVTGKAANFVHMDTAQKKLQEFNARRKLKAAVKAVVASSRLGSASSSHGSIQESHKASRDPSPIQDGNEDMKAIPEGEKIQGDGAQAAVKGAQAELMKVQALEKVKGADINAEEAPKMVPKAVEDGIKVADLELEEGLAEEKLKTVEEAAAPREGQGSSAVGFEVPQQDVILPEY
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Molecular Weight
51.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The KCC4 (K+ Cl- cotransporter 4) protein is part of the SLC12 family of transporters, playing a crucial role in regulating cellular ion homeostasis and volume. It is primarily expressed in various tissues, including the kidney, brain, and heart, where it contributes to the reabsorption of potassium and chloride ions, essential for maintaining electrolyte balance. Dysregulation of KCC4 has been implicated in several physiological and pathological conditions, such as hypertension, cystic fibrosis, and neurological disorders. Recent research has focused on the structural and functional characterization of KCC4, utilizing techniques such as cryo-electron microscopy and X-ray crystallography to elucidate its transport mechanism and substrate specificity. Understanding the molecular basis of KCC4 activity could provide insights into its role in disease processes and offer potential therapeutic targets. Furthermore, the investigation of KCC4 as a recombinant protein has opened avenues for the development of innovative drug delivery systems and biosensors, enhancing our ability to monitor and manipulate ion transport in various biological settings. Overall, the study of KCC4 is pivotal in advancing our knowledge of cellular transport mechanisms and their implications for human health.
