Analytical Data
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Gene name
PARP14
- Application
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Alternative Names
PARP14；BAL2；KIAA1268；Protein mono-ADP-ribosyltransferase PARP14
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q460N5
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Expression Region
1470-1801aa
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AA Sequence
MHHHHHHGGGSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKW RNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAE ISMLEGAVLDIRYGVSRIAYSKDFETLVDFLSKLPEMLKMFKDRLCHKTY LNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKY LKSSKYIAWPLQGWQATFGGGDHPPKSDPENLYFQGGGEFKEYQELNELQ KKLNNISLDHKRPLIKVLGISRDVMQARDEIEAMIKRVRLAKEQESRADC ISEFIEWQYNDNNTSHCFNKMTNLKLEDARREKKKTVDVKINHRHYTVNL NTYTATDTKGHSLSVQRLTKSKVDIPAHWSDKQQNFCVVELLPSDPEYNT VASKFNQTCSHFRIEKIERIQNPDLWNSYQAKKKTMDAKNGQTMNEKQLF HGTDAGSVPHVNRNGFNRSYAGKNAVAYGKGTYFAVNANYSANDTYSRPD ANGRKHVYVRVLTGIYTHGNHSLIVPPSKNPQNPTDLYDTVTDNVHHPSL FVAFYDYQAYPEYLITFRK
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Molecular Weight
67 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PARP14 (Poly(ADP-ribose) polymerase 14) is a member of the PARP family, which is critically involved in the regulation of various cellular processes, including DNA repair, apoptosis, and immune responses. Unlike its better-known relatives, PARP14 functions primarily as a mono-ADP-ribosyltransferase and is implicated in the regulation of gene expression through its interaction with transcription factors. Recent studies have highlighted its role in modulating inflammatory responses and in the development of certain cancers, making it a potential target for therapeutic intervention. The recombinant expression of PARP14 protein allows for detailed structural and functional studies, facilitating the understanding of its biological role and the underlying mechanisms by which it influences cellular pathways. By producing and characterizing PARP14 in a controlled environment, researchers aim to elucidate its enzymatic activity, identify potential inhibitors, and explore its interactions with other proteins. Such research could lead to novel strategies for the treatment of diseases related to dysregulated PARP14 activity, including autoimmune disorders and malignancies.
