Analytical Data
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基因名
yscF
- Application
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别名
yscF;yscF;Type 3 secretion system needle filament Protein
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q01247
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表达区间
1-87aa
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氨基酸序列
MSNFSGFTKGNDIADLDAVAQTLKKPADDANKAVNDSIAALKDTPDNPALLADLQHSINKWSVIYNISSTIVRSMKDLMQGILQKFP
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分子量
9.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The yscF gene, part of the type III secretion system (T3SS) in various pathogens, plays a critical role in the virulence and pathogenicity of several bacteria. This gene encodes a small protein that is believed to be involved in the assembly and stability of the T3SS structure, which is crucial for the translocation of effector proteins into host cells. Understanding yscF and its encoded protein is vital for dissecting the mechanisms of bacterial infection and the host-pathogen interaction. Several studies have highlighted that manipulating or targeting yscF can influence the pathogenicity of bacteria, making it a potential candidate for developing novel therapeutic strategies. Research focusing on the recombinant form of yscF protein aims to elucidate its function, structural properties, and interactions within the T3SS. Additionally, recombinant yscF protein can serve as a valuable tool for generating antibodies and understanding the immune response elicited during infection. Investigating yscF protein not only enhances our understanding of bacterial virulence mechanisms but also contributes to the broader field of microbiology and infectious disease research. The potential to develop vaccines or inhibitors targeting yscF offers promising avenues for combating bacterial infections that rely on T3SS for their pathogenic effects.












