Analytical Data
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Gene name
brkA
- Application
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Species
Bordetella pertussis
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q45340
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Expression Region
1-1010aa
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Molecular Weight
110.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The BrkA protein, derived from the pathogenic bacterium *Brucella spp.*, plays a crucial role in the organism's virulence and immune evasion mechanisms. As a member of the ATP-binding cassette (ABC) transporter family, BrkA is implicated in the intracellular survival of *Brucella*, enabling it to evade host immune responses and replicate within host cells. Research into BrkA has garnered significant attention due to its potential as a target for vaccine development and treatment strategies against brucellosis, a zoonotic disease affecting both livestock and humans. Understanding the structure and function of BrkA through recombinant protein expression can provide insights into its role in pathogenesis and help identify potential therapeutic interventions. Previous studies have shown that BrkA can modulate host cell signaling pathways, emphasizing its significance in the bacteria's ability to manipulate the host environment for survival. Additionally, the production of recombinant BrkA protein allows for the examination of antibody responses in vaccinated animals, thus aiding in the development of diagnostic tools and vaccines. Overall, research on BrkA not only enhances our understanding of *Brucella* biology but also contributes to broader efforts in infectious disease control and prevention.
