Analytical Data
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基因名
rpmE
- Application
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别名
rpmE；Large ribosomal subunit Protein bL31
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0A7M9
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表达区间
1-70aa
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氨基酸序列
MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFFTGKQRDVATGGRVDRFNKRFNIPGSK
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分子量
34.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RpmE is a crucial protein found in bacteria that plays a significant role in the assembly of the ribosomal 50S subunit, a fundamental component of the ribosome responsible for protein synthesis. Research on RpmE has gained traction due to its potential implications in understanding bacterial ribosome function and the development of novel antibiotics. Given the rising threat of antibiotic resistance, targeting ribosomal proteins such as RpmE presents a promising strategy for designing new therapeutic agents. The protein is known to interact with other ribosomal proteins and rRNA, contributing to the intricate process of ribosome maturation. Studies have demonstrated that mutations in the rpmE gene can lead to defects in ribosome assembly, which in turn affects bacterial growth and viability, highlighting its essential role in cellular biology. Moreover, the conservation of RpmE across various bacterial species suggests that it may serve as a universal target for antibiotic development. Investigating the structure and function of RpmE can provide insights into the fundamental mechanisms of ribosome assembly and offer opportunities for innovative approaches in combating bacterial infections. Overall, the study of RpmE and its dynamics within the ribosomal environment is poised to contribute significantly to both microbiology and antibiotic research.
