Analytical Data
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基因名
rpmF
- Application
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别名
rpmF；Large ribosomal subunit Protein bL32
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0A7N4
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表达区间
2-57aa
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氨基酸序列
AVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHITADGYYRGRKVIAK
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分子量
33.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RpmF is a highly conserved ribosomal protein that plays a crucial role in the assembly and function of the ribosome in various bacterial species. As a member of the 50S subunit, RpmF is essential for proper protein synthesis, as it influences ribosomal structure and stability. Studies have shown that mutations or deletions in the rpmF gene can lead to defects in translation and, consequently, bacterial growth and viability. Given the rising concern of antibiotic resistance, RpmF has emerged as a potential target for novel antimicrobial agents. Researchers have been investigating its structure and function to understand how it interacts with ribosomal RNA and other ribosomal proteins. Furthermore, the high conservation of RpmF across species suggests that it may serve as a universal target for antibiotics, which could lead to the development of broad-spectrum drugs. The recombinant expression of RpmF protein allows for the exploration of its biochemical properties, facilitating assays that evaluate its role in ribosome assembly and function. Such studies not only enhance our knowledge of bacterial ribosomes but also contribute to the design of innovative therapeutic strategies that could circumvent existing drug resistance mechanisms by targeting ribosomal components like RpmF.
