Analytical Data
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Gene name
rpmF
- Application
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Alternative Names
rpmF；Large ribosomal subunit Protein bL32
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0A7N4
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Expression Region
2-57aa
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AA Sequence
AVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHITADGYYRGRKVIAK
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Molecular Weight
33.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RpmF is a highly conserved ribosomal protein that plays a crucial role in the assembly and function of the ribosome in various bacterial species. As a member of the 50S subunit, RpmF is essential for proper protein synthesis, as it influences ribosomal structure and stability. Studies have shown that mutations or deletions in the rpmF gene can lead to defects in translation and, consequently, bacterial growth and viability. Given the rising concern of antibiotic resistance, RpmF has emerged as a potential target for novel antimicrobial agents. Researchers have been investigating its structure and function to understand how it interacts with ribosomal RNA and other ribosomal proteins. Furthermore, the high conservation of RpmF across species suggests that it may serve as a universal target for antibiotics, which could lead to the development of broad-spectrum drugs. The recombinant expression of RpmF protein allows for the exploration of its biochemical properties, facilitating assays that evaluate its role in ribosome assembly and function. Such studies not only enhance our knowledge of bacterial ribosomes but also contribute to the design of innovative therapeutic strategies that could circumvent existing drug resistance mechanisms by targeting ribosomal components like RpmF.
