Analytical Data
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Gene name
TRIM28
- Application
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Alternative Names
TRIM28；KAP1；RNF96；TIF1B；Transcription intermediary factor 1-beta
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q13263
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Expression Region
22-291aa
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AA Sequence
PGEGSAGGEKRSTAPSAAASASASAAASSPAGGGAEALELLEHCGVCRERLRPEREPRLLPCLHSACSACLGPAAPAAANSSGDGGAAGDGTVVDCPVCKQQCFSKDIVENYFMRDSGSKAATDAQDANQCCTSCEDNAPATSYCVECSEPLCETCVEAHQRVKYTKDHTVRSTGPAKSRDGERTVYCNVHKHEPLVLFCESCDTLTCRDCQLNAHKDHQYQFLEDAVRNQRKLLASLVKRLGDKHATLQKSTKEVRSSIRQVSDVQKRV
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Molecular Weight
60.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
TRIM28, also known as KAP1 (KRAB-associated protein 1), is a critical member of the TRIM (tripartite motif) family of proteins that plays a significant role in various biological processes, including transcriptional regulation, DNA damage response, and cellular differentiation. This protein is particularly known for its function as a transcriptional co-repressor, where it interacts with KRAB zinc-finger proteins to silence gene expression. Research on TRIM28 has revealed its involvement in various cellular functions, such as embryonic stem cell maintenance, viral restriction, and modulation of immune responses. Additionally, TRIM28 has been implicated in cancer biology, with its expression levels often associated with tumor progression and poor prognosis. Due to its multifaceted roles, TRIM28 presents a promising target for therapeutic interventions in cancer and other diseases. Emerging studies are focused on the mechanistic understanding of TRIM28’s interactions with other proteins and its regulatory pathways, which may provide insights into its potential as a biomarker or therapeutic target. The generation of TRIM28 recombinant proteins allows for detailed structural and functional analyses, facilitating the exploration of its roles in various cellular contexts and disease states. As research continues to unfold, the comprehensive understanding of TRIM28's mechanisms could lead to novel therapeutic strategies that leverage its functions in gene regulation and cellular homeostasis.
