Analytical Data
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Gene name
PGD
- Application
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Alternative Names
PGD；PGDH；6-phosphogluconate dehydrogenase. decarboxylating
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P52209
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Expression Region
1-483aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MAQADIALIG LAVMGQNLIL NMNDHGFVVC AFNRTVSKVD DFLANEAKGT KVVGAQSLKE MVSKLKKPRR IILLVKAGQA VDDFIEKLVP LLDTGDIIID GGNSEYRDTT RRCRDLKAKG ILFVGSGVSG GEEGARYGPS LMPGGNKEAW PHIKTIFQGI AAKVGTGEPC CDWVGDEGAG HFVKMVHNGI EYGDMQLICE AYHLMKDVLG MAQDEMAQAF EDWNKTELDS FLIEITANIL KFQDTDGKHL LPKIRDSAGQ KGTGKWTAIS ALEYGVPVTL IGEAVFARCL SSLKDERIQA SKKLKGPQKF QFDGDKKSFL EDIRKALYAS KIISYAQGFM LLRQAATEFG WTLNYGGIAL MWRGGCIIRS VFLGKIKDAF DRNPELQNLL LDDFFKSAVE NCQDSWRRAV STGVQAGIPM PCFTTALSFY DGYRHEMLPA SLIQAQRDYF GAHTYELLAK PGQFIHTNWT GHGGTVSSSS YNA
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Molecular Weight
55 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PGD, or Phosphogluconate Dehydrogenase, is an important enzyme in the pentose phosphate pathway, a metabolic pathway parallel to glycolysis. This enzyme plays a crucial role in cellular metabolism, particularly in the generation of NADPH, which is vital for biosynthetic reactions and maintaining cellular redox balance. The study of PGD recombinant protein has garnered attention due to its potential applications in both clinical and biotechnological fields. In cancer research, altered PGD activity has been linked to tumorigenesis and cancer cell proliferation, making it a target for therapeutic interventions. Additionally, its role in redox homeostasis and anabolic processes underscores its importance in various metabolic disorders. Advances in recombinant DNA technology have enabled the production of PGD in heterologous systems, allowing for detailed structural and functional studies of the enzyme. This research is not only aimed at understanding the fundamental biology of PGD but also at exploring its potential as a biomarker for disease diagnosis and a target for drug development. Moreover, the characterization of PGD recombinant proteins can help in the design of enzyme activators or inhibitors, paving the way for new therapeutic strategies. As such, PGD represents a promising area of research with implications for understanding metabolic diseases, cancer biology, and the development of biotechnological applications.
