Analytical Data
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Gene name
CHD4
- Application
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Alternative Names
CHD4；Chromodomain-helicase-DNA-binding Protein 4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q14839
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Expression Region
1681-1912aa
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AA Sequence
KDLNDEKQKK NIKQRFMFNI ADGGFTELHS LWQNEERAAT VTKKTYEIWH RRHDYWLLAG IINHGYARWQ DIQNDPRYAI LNEPFKGEMN RGNFLEIKNK FLARRFKLLE QALVIEEQLR RAAYLNMSED PSHPSMALNT RFAEVECLAE SHQHLSKESM AGNKPANAVL HKVLKQLEEL LSDMKADVTR LPATIARIPP VAVRLQMSER NILSRLANRA PEPTPQQVAQ QQ
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Molecular Weight
32 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CHD4, a member of the chromodomain helicase DNA-binding (CHD) family, plays a crucial role in chromatin remodeling and gene regulation, making it a significant focus in molecular biology and cancer research. It is a key component of the NuRD (Nucleosome Remodeling and Deacetylase) complex, which is involved in various cellular processes, including differentiation, proliferation, and the maintenance of genomic stability. Dysregulation of CHD4 has been implicated in several types of cancer and developmental disorders, highlighting the importance of understanding its function and regulatory mechanisms. Recent studies have shown that CHD4 interacts with various transcription factors and co-regulators, influencing gene expression profiles in response to environmental signals. Furthermore, the development of CHD4 recombinant proteins has facilitated the elucidation of its structural and functional properties, allowing researchers to investigate its role in chromatin dynamics and epigenetic regulation. Through techniques such as X-ray crystallography and cryo-electron microscopy, insights into the conformational changes of CHD4 during nucleosome remodeling have been gained. Overall, the study of CHD4 recombinant proteins not only enhances our understanding of its molecular functions but also opens avenues for therapeutic interventions targeting CHD4-related pathways in cancer and other diseases.
