Analytical Data
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Gene name
KIF18B
- Application
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Species
Human
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q86Y91
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Expression Region
1-355aa
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Molecular Weight
46.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
KIF18B is a member of the kinesin superfamily of motor proteins, which play a critical role in intracellular transport and mitotic processes. This protein is particularly involved in the regulation of spindle dynamics during cell division, where it aids in the accurate alignment and segregation of chromosomes. Studies have shown that KIF18B is essential for proper mitotic progression, and its dysfunction can lead to chromosomal instability, a hallmark of various cancers. The interest in KIF18B as a target for therapeutic intervention arises from its specific expression in certain cancer types, suggesting that manipulating its activity could potentially improve cancer treatment outcomes. To investigate the functional mechanisms and interactions of KIF18B, researchers have focused on producing recombinant KIF18B proteins. This involves various expression systems, such as bacterial, yeast, or insect cells, to obtain sufficient quantities of the protein for biochemical and biophysical analyses. Understanding KIF18B's structure, function, and regulation could provide valuable insights into its role in cancer biology and offer opportunities for developing novel therapeutic strategies. As such, the study of recombinant KIF18B not only enhances our understanding of fundamental cellular processes but also opens doors for innovative approaches in cancer treatment, highlighting the importance of this motor protein in both basic research and clinical applications.
