Analytical Data
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Gene name
GCNF
- Application
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Alternative Names
GCNF；GCNF；Nuclear receptor subfamily 6 group A member 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q15406
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Expression Region
1-480aa
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AA Sequence
MERDEPPPSG GGGGGGSAGF LEPPAALPPP PRNGFCQDEL AELDPGTISV SDDRAEQRTC LICGDRATGL HYGIISCEGC KGFFKRSICN KRVYRCSRDK NCVMSRKQRN RCQYCRLLKC LQMGMNRKAI REDGMPGGRN KSIGPVQISE EEIERIMSGQ EFEEEANHWS NHGDSDHSSP GNRASESNQP SPGSTLSSSR SVELNGFMAF REQYMGMSVP PHYQYIPHLF SYSGHSPLLP QQARSLDPQS YSLIHQLLSA EDLEPLGTPM LIEDGYAVTQ AELFALLCRL ADELLFRQIA WIKKLPFFCE LSIKDYTCLL SSTWQELILL SSLTVYSKQI FGELADVTAK YSPSDEELHR FSDEGMEVIE RLIYLYHKFH QLKVSNEEYA CMKAINFLNQ DIRGLTSASQ LEQLNKRYWY ICQDFTEYKY THQPNRFPDL MMCLPEIRYI AGKMVNVPLE QLPLLFKVVL HSCKTSVGKE
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GCNF (Germ Cell Nuclear Factor) is a transcription factor crucial for germ cell development and reproductive biology, playing a significant role in the regulation of genes essential for gametogenesis. Its expression is tightly regulated during embryonic development and is localized primarily in the nucleus of germ cells, indicating its potential involvement in maintaining pluripotency and promoting the differentiation of these cells into mature gametes. Recent studies have highlighted GCNF's pivotal role in preventing premature differentiation of germ cells, ensuring proper embryonic development and fertility. The understanding of GCNF's functions has expanded with advancements in molecular biology techniques, leading to a keen interest in exploring its interactions with other regulatory proteins and its downstream targets. Furthermore, the recombinant expression of GCNF protein has become a focal point for studying its biochemical properties and functional mechanisms. Investigating GCNF at the protein level not only elucidates its specific roles in germ cell lineage but also provides insights into potential therapeutic applications, particularly in reproductive medicine and regenerative biology. As research continues, the characterization of GCNF as a recombinant protein will pave the way for innovative strategies to manipulate germ cell development, which may ultimately lead to novel treatments for infertility and other reproductive disorders.
