Analytical Data
-
Gene name
Grb10
- Application
-
Alternative Names
Grb10；GRBIR；KIAA0207；Growth factor receptor-bound Protein 10
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q13322-3
-
Expression Region
1-536aa
-
AA Sequence
MNASLESLYSACSMQSDTVPLLQNGQHARSQPRASGPPRSIQPQVSPRQR VQRSQPVHILAVRRLQEEDQQFRTSSLPAIPNPFPELCGPGSPPVLTPGS LPPSQAAAKQDVKVFSEDGTSKVVEILADMTARDLCQLLVYKSHCVDDNS WTLVEHHPHLGLERCLEDHELVVQVESTMASESKFLFRKNYAKYEFFKNP MNFFPEQMVTWCQQSNGSQTQLLQNFLNSSSCPEIQGFLHVKELGKKSWK KLYVCLRRSGLYCSTKGTSKEPRHLQLLADLEDSNIFSLIAGRKQYNAPT DHGLCIKPNKVRNETKELRLLCAEDEQTRTCWMTAFRLLKYGMLLYQNYR IPQQRKALLSPFSTPVRSVSENSLVAMDFSGQTGRVIENPAEAQSAALEE GHAWRKRSTRMNILGSQSPLHPSTLSTVIHRTQHWFHGRISREESHRIIK QQGLVDGLFLLRDSQSNPKAFVLTLCHHQKIKNFQILPCEDDGQTFFSLD DGNTKFSDLIQLVDFYQLNKGVLPCKLKHHCIRVAL
-
Molecular Weight
87 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Grb10 (Growth factor receptor-bound protein 10) is an adaptor protein that plays a significant role in various cellular processes, including growth, metabolism, and signal transduction. Research into Grb10 has gained momentum because of its involvement in insulin signaling pathways and its potential implications in metabolic disorders like obesity and type 2 diabetes. Furthermore, Grb10 has been shown to interact with various receptor tyrosine kinases, which are crucial for cell signaling in response to growth factors. The interest in Grb10 is also fueled by its unique regulatory functions, particularly in the context of imprinted genes and their effects on developmental biology. The study of Grb10 recombinant proteins is vital for elucidating its structural and functional characteristics, providing insights into its molecular mechanisms. By producing and analyzing recombinant Grb10, researchers aim to uncover its detailed role in cellular signaling and its potential as a therapeutic target. Moreover, understanding the dynamics of Grb10's interactions can open avenues for novel drug developments aimed at treating metabolic diseases and conditions related to aberrant signaling pathways. Overall, Grb10's intricate involvement in essential biological processes renders it a key focus in both basic and translational research, underscoring the necessity for further studies on its recombinant forms to fully exploit its potential in biomedical applications.
