Analytical Data
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Gene name
ASMT
- Application
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Alternative Names
ASMT；Acetylserotonin O-methyltransferase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P46597-2
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Expression Region
1-298aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSSEDQAYR LLNDYANGFM VSQVLFAACE LGVFDLLAEA PGPLDVAAVA AGVRASAHGT ELLLDICVSL KLLKVETRGG KAFYRNTELS SDYLTTVSPT SQCSMLKYMG RTSYRCWGHL ADAVREGRNQ YLETFGVPAE ELFTAIYRSE GERLQFMQAL QEVWSVNGRS VLTAFDLSVF PLMCDLGGDF FKDPLPEADL YILARVLHDW ADGKCSHLLE RIYHTCKPGG GILVIESLLD EDRRGPLLTQ LYSLNMLVQT EGQERTPTHY HMLLSSAGFR DFQFKKTGAI YDAILARK
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Molecular Weight
35 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ASMT (acetylserotonin O-methyltransferase) is an important enzyme involved in the biosynthesis of melatonin, a hormone that regulates sleep-wake cycles and circadian rhythms in various organisms. The significance of studying ASMT arises from its role in modulating physiological processes influenced by melatonin, including sleep disorders, seasonal affective disorder, and reproductive functions. As global interest in the therapeutic potential of melatonin continues to grow, understanding the structure and function of ASMT becomes crucial. Research on recombinant ASMT has gained momentum, leveraging genetic engineering techniques to produce the enzyme in a controlled manner. This recombinant approach allows for extensive characterization of ASMT's enzymatic properties, substrate specificity, and regulatory mechanisms, facilitating insights into its biological functions. Furthermore, generating ASMT in a laboratory setting opens avenues for potential applications in drug development and supplements aimed at enhancing melatonin levels. Overall, the study of recombinant ASMT not only enriches our comprehension of melatonin metabolism but also presents opportunities for innovative treatments addressing sleep and mood disorders, thereby highlighting the enzyme's vital role in health and well-being.
