Analytical Data
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Gene name
nosZ
- Application
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Alternative Names
N(2)OR N2O reductase
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Species
Paracoccus denitrificans
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Source
E. coli
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Tag
N- His-SUMO
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q51705
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Expression Region
58-553aa
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Molecular Weight
71.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of the nosZ gene and its recombinant protein is crucial in understanding the biological process of denitrification, a key environmental process that converts nitrate to nitrogen gas, thereby mitigating nitrogen pollution in ecosystems. nosZ encodes for a nitrous oxide reductase enzyme, which plays a pivotal role in the final step of denitrification, transforming nitrous oxide (a potent greenhouse gas) into harmless nitrogen gas. Given the increasing concerns about climate change and the contribution of nitrous oxide to atmospheric warming, elucidating the structure and function of the nosZ protein is of significant interest. Research efforts have focused on cloning the nosZ gene from various microbial sources, followed by expression in suitable host systems to produce recombinant proteins for biochemical characterization. This has facilitated studies into the enzymatic mechanisms, substrate specificity, and potential applications in bioremediation and sustainable agriculture. Additionally, the exploration of nosZ in different environmental contexts, such as soils and aquatic systems, has provided insights into its ecological significance and its role in nitrogen cycling. Understanding the nuances of nosZ protein interactions and its regulation under different environmental conditions can lead to improved strategies for enhancing denitrification processes in agricultural practices, ultimately contributing to reduced greenhouse gas emissions and improved soil health. Overall, research on nosZ recombinant protein represents a vital intersection of microbiology, environmental science, and biotechnology, addressing pressing global environmental challenges.
