Analytical Data
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Gene name
Twist1
- Application
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Alternative Names
(M-twist)
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Species
Mouse
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Source
E. coli
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Tag
N- His-KSI
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P26687
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Expression Region
1-206aa
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Molecular Weight
36.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Twist1 is a basic helix-loop-helix (bHLH) transcription factor that plays a critical role in various biological processes, including embryogenesis, tissue differentiation, and tumor progression. It is primarily known for its function in mesoderm formation and the regulation of epithelial-to-mesenchymal transition (EMT), a process fundamental to organ development and cancer metastasis. Dysregulation of Twist1 expression has been linked to several malignancies, suggesting its potential as a target for therapeutic intervention. The study of recombinant Twist1 protein has gained traction in recent years, as it allows for a better understanding of its molecular mechanisms and interactions. Researchers have sought to produce recombinant Twist1 to investigate its role in gene expression regulation, cell migration, and invasion, particularly in cancerous cells. By utilizing various expression systems, scientists can produce large quantities of functional Twist1 protein, which can then be used in in vitro assays to analyze its activity and discover potential binding partners. Furthermore, the exploration of Twist1's signaling pathways and its interaction with other transcription factors or co-factors provides insights into its multifaceted roles in normal development and disease. Understanding Twist1's biochemical properties and regulatory mechanisms is crucial not only for elucidating its contribution to developmental biology but also for identifying novel therapeutic strategies in cancer treatment, where modulating its function may inhibit tumor progression and improve patient outcomes. This ongoing research underscores the importance of Twist1 in both fundamental biology and clinical applications, highlighting its promise as a significant biomarker and therapeutic target in oncology.
