Analytical Data
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Gene name
ANAPC13
- Application
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Alternative Names
ANAPC13；Anaphase-promoting complex subunit 13
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BS18
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Expression Region
1-74aa
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AA Sequence
MASMTGGQQM GRGSHMDSEV QRDGRILDLI DDAWREDKLP YEDVAIPLNE LPEPEQDNGG TTESVKEQEM KWTDLALQYL HENVPPIGN
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Molecular Weight
10 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ANAPC13, a crucial component of the Anaphase-Promoting Complex/Cyclosome (APC/C), plays a vital role in the regulation of the cell cycle by mediating the ubiquitination and subsequent degradation of cell cycle proteins. The APC/C is a multi-subunit E3 ubiquitin ligase that orchestrates the transition from metaphase to anaphase by targeting key regulatory proteins, such as cyclins and other cell cycle inhibitors, for proteasomal degradation. This process ensures the proper timing of chromosome segregation and the overall fidelity of cell division. Dysregulation of APC/C activity, particularly involving subunits like ANAPC13, has been implicated in various cancers, as aberrant cell cycle progression can lead to uncontrolled cellular proliferation. Furthermore, understanding the structure and function of ANAPC13 is critical for elucidating the mechanisms of APC/C-mediated protein degradation and its broader implications in cancer biology and therapeutics. Recent research has focused on the biochemistry of ANAPC13, including its interactions with other APC/C subunits and substrates, as well as its post-translational modifications. By studying recombinant ANAPC13 proteins in vitro, researchers aim to reveal insights into the regulatory mechanisms of APC/C, which may pave the way for the development of targeted cancer therapies that could restore normal cell cycle control or exploit vulnerabilities in cancer cells resulting from APC/C dysfunction. The integration of structural biology techniques, such as X-ray crystallography and cryo-electron microscopy, with functional assays will contribute to a comprehensive understanding of ANAPC13's role within the APC/C complex. This research is pivotal in advancing our knowledge of cell cycle regulation and its implications in oncogenesis.
