Analytical Data
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Gene name
Aldh2
- Application
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Alternative Names
Aldh2；ALDM；Aldehyde dehydrogenase. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P05091
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Expression Region
18-517aa
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AA Sequence
MSAAATQAVP APNQQPEVFC NQIFINNEWH DAVSRKTFPT VNPSTGEVIC QVAEGDKEDV DKAVKAARAA FQLGSPWRRM DASHRGRLLN RLADLIERDR TYLAALETLD NGKPYVISYL VDLDMVLKCL RYYAGWADKY HGKTIPIDGD FFSYTRHEPV GVCGQIIPWN FPLLMQAWKL GPALATGNVV VMKVAEQTPL TALYVANLIK EAGFPPGVVN IVPGFGPTAG AAIASHEDVD KVAFTGSTEI GRVIQVAAGS SNLKRVTLEL GGKSPNIIMS DADMDWAVEQ AHFALFFNQG QCCCAGSRTF VQEDIYDEFV ERSVARAKSR VVGNPFDSKT EQGPQVDETQ FKKILGYINT GKQEGAKLLC GGGIAADRGY FIQPTVFGDV QDGMTIAKEE IFGPVMQILK FKTIEEVVGR ANNSTYGLAA AVFTKDLDKA NYLSQALQAG TVWVNCYDVF GAQSPFGGYK MSGSGRELGE YGLQAYTEVK TVTVKVPQKN S
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Molecular Weight
55 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Aldehyde dehydrogenase 2 (Aldh2) is a crucial enzyme involved in the detoxification of aldehydes, particularly acetaldehyde, which is a byproduct of alcohol metabolism. Deficiencies in Aldh2 activity are linked to various health issues, including alcohol intolerance and increased risk for alcohol-related diseases, such as certain cancers and cardiovascular conditions. Genetic variants of the Aldh2 gene, particularly the common variant found in East Asian populations (Aldh2*2), result in significantly reduced enzyme activity, leading to the accumulation of toxic aldehydes in the body. This has spurred research into recombinantly expressing Aldh2 to study its properties, regulatory mechanisms, and potential therapeutic applications. By producing recombinant Aldh2 protein, researchers can investigate its structure-function relationship, enzyme kinetics, and interactions with other metabolic pathways. Moreover, recombinant Aldh2 can serve as a valuable tool in drug development, where enhancing its activity may offer protective effects against aldehyde-mediated toxicity. Understanding the molecular mechanisms of Aldh2 can thus provide insights into the physiological role of this enzyme, inform strategies to mitigate alcohol-related damage, and advance therapeutic approaches for related diseases.
