Analytical Data
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基因名
ACTR3
- Application
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别名
ACTR3；ARP3；Actin-related Protein 3
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P61158
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表达区间
1-418aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHMGSMAGRLPACVVDCGTGYTKLGYAGNTEP QFIIPSCIAIKESAKVGDQAQRRVMKGVDDLDFFIGDEAIEKPTYATKWP IRHGIVEDWDLMERFMEQVIFKYLRAEPEDHYFLLTEPPLNTPENREYTA EIMFESFNVPGLYIAVQAVLALAASWTSRQVGERTLTGTVIDSGDGVTHV IPVAEGYVIGSCIKHIPIAGRDITYFIQQLLRDREVGIPPEQSLETAKAV KERYSYVCPDLVKEFNKYDTDGSKWIKQYTGINAISKKEFSIDVGYERFL GPEIFFHPEFANPDFTQPISEVVDEVIQNCPIDVRRPLYKNIVLSGGSTM FRDFGRRLQRDLKRTVDARLKLSEELSGGRLKPKPIDVQVITHHMQRYAV WFGGSMLASTPEFYQVCHTKKDYEEIGPSICRHNPVFGVMS
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分子量
50 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ACTR3, also known as ARP3, is a key component of the ARP2/3 complex, which plays a vital role in the regulation of the actin cytoskeleton. The actin cytoskeleton is essential for various cellular processes, including cell motility, division, and maintenance of shape. The ARP2/3 complex is responsible for promoting the nucleation of new actin filaments, thereby facilitating the formation of branched actin networks. Dysregulation of actin dynamics is associated with several diseases, including cancer metastasis and neurodegenerative disorders. Research on ACTR3's recombinant protein focuses on its structural and functional properties, enabling scientists to understand the mechanisms by which the ARP2/3 complex interacts with actin filaments. Additionally, elucidating the role of ACTR3 in cellular processes can provide insights into potential therapeutic targets for diseases rooted in actin dysfunction. The study of recombinant ACTR3 involves techniques such as X-ray crystallography and cryo-electron microscopy, helping to detail its interactions with other proteins and actin. Given its importance in numerous cellular functions and disease contexts, ACTR3 remains a significant target of investigation in cell biology and biophysics, contributing to our understanding of cytoskeletal dynamics and potential interventions in actin-related pathologies.
