Analytical Data
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Gene name
ACTN1
- Application
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Alternative Names
ACTN1；Alpha-actinin-1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P12814-3
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Expression Region
1-249aa
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AA Sequence
MGSSHHHHHHSSGLVPRGSHMGSEFMDHYDSQQTNDYMQPEEDWDRDLLL DPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGE RLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLG MIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKD GLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAE DIVGTARPDEKAIMTYVSSFYHAF
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Molecular Weight
31 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ACTN1, or alpha-actinin-1, is a pivotal actin-binding protein that plays a crucial role in the organization of the cytoskeleton, muscle contraction, and signal transduction. It is predominantly expressed in striated muscles, where it contributes to the structural integrity of sarcomeres by cross-linking actin filaments. Mutations in the ACTN1 gene have been linked to various myopathies, highlighting its significance in muscle health and function. Recent studies have focused on the recombinant expression of ACTN1 to elucidate its biophysical properties, interaction dynamics with other sarcomeric proteins, and its role in muscle diseases. Understanding the structural and functional aspects of ACTN1 at a molecular level can provide insights into the pathogenesis of myopathies associated with its dysfunction. The use of recombinant protein techniques allows researchers to produce large quantities of ACTN1 for biochemical assays and structural studies, thereby advancing our knowledge of muscle biology and offering potential therapeutic avenues for ACTN1-related disorders. Enhanced comprehension of this protein's attributes and interactions with actin and other cytoskeletal components will pave the way for targeted approaches in treating muscular diseases stemming from ACTN1 mutations.
