Analytical Data
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Gene name
ABHD14B
- Application
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Alternative Names
ABHD14B；CIB；Putative Protein-lysine deacylase ABHD14B
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96IU4
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Expression Region
1-210aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAASVE QREGTIQVQG QALFFREALP GSGQARFSVL LLHGIRFSSE TWQNLGTLHR LAQAGYRAVA IDLPGLGHSK EAAAPAPIGE LAPGSFLAAV VDALELGPPV VISPSLSGMY SLPFLTAPGS QLPGFVPVAP ICTDKINAAN YASVKTPALI VYGDQDPMGQ TSFEHLKQLP NHRVLIMKGA GHPCYLDKPE EWHTGLLDFL QGLQ
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Molecular Weight
25 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ABHD14B (Abhydrolase Domain Containing 14B) is a member of the α/β hydrolase fold family, which is known for its involvement in various biological processes, including lipid metabolism, signaling, and cellular communication. The enzyme is particularly interesting due to its potential role in endocannabinoid signaling pathways, where it may influence the degradation of lipid signaling molecules like anandamide. Research has shown that ABHD14B exhibits significant substrate specificity, suggesting its potential as a therapeutic target in conditions like pain, mood disorders, and neurodegenerative diseases. Given its functional implications, the production and characterization of recombinant ABHD14B protein have become critical for understanding its biochemical properties, regulatory mechanisms, and interactions with other cellular components. Recent advances in recombinant DNA technology allow for the expression of ABHD14B in heterologous systems, enabling detailed enzymatic assays and structural studies. This research not only aids in elucidating the enzyme's function but also opens avenues for drug discovery, as modulating its activity could provide novel strategies for treating diseases linked to dysfunctional lipid signaling. Overall, the study of ABHD14B recombinant protein is vital for both basic science and potential clinical applications, highlighting the importance of continuing to investigate the complexities of lipid metabolism and signaling pathways in health and disease.
