Analytical Data
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基因名
Papain
- Application
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别名
Papaya proteinase I Short name:PPI
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种属
Carica papaya
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表达系统
E. coli
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标签
N- His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P00784
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表达区间
134-345aa
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分子量
27.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Papain is a proteolytic enzyme derived from the papaya fruit (Carica papaya) and has garnered significant interest in both scientific research and industrial applications due to its ability to hydrolyze peptide bonds in proteins. Historically, papain has been extensively studied for its potential health benefits, including its role in digestion and its anti-inflammatory properties. The interest in recombinant papain stems from the limitations associated with extracting the enzyme from natural sources, including issues of yield, activity, and contamination with other proteases. Recombinant DNA technology allows for the production of papain in a more controlled and efficient manner, enabling researchers to engineer the enzyme for enhanced stability, specificity, and activity under various conditions. Additionally, recombinant papain can be used in applications such as food processing, medicine, and biochemistry, where its proteolytic properties are particularly valuable. As a result, the study of recombinant papain not only aims to optimize its biochemical properties but also to explore its potential therapeutic uses, thus contributing to advancements in biotechnology and pharmaceutical sciences. The development of recombinant papain represents a promising avenue for both fundamental research and practical applications, making it an important focus within enzyme engineering and protein biotechnology.
