Analytical Data
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Gene name
ksdD
- Application
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Alternative Names
3-keto-Delta(4)-steroid Delta(1)-dehydrogenase;KSDD;3-oxo-Delta(4)-steroid 1-dehydrogenase;KSTD
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Species
Mycolicibacterium smegmatis
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
A0R4S9
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Expression Region
1-569aa
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Molecular Weight
68.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
KSDD (Kinetoplastida Surface-Defined Domain) recombinant proteins have garnered significant interest in the field of molecular biology and parasitology due to their potential applications in vaccine development and therapeutic interventions against diseases caused by Kinetoplastida, such as African sleeping sickness and Chagas disease. These proteins are derived from the unique mid-outer membrane structures of trypanosomes and other related parasites, which play crucial roles in host-pathogen interactions. The study of KSDD recombinant proteins aims to elucidate their structure, function, and immunogenic properties, thereby providing insights into the immune evasion mechanisms of these parasites. Recent advances in protein engineering and expression systems have enabled researchers to produce KSDD recombinant proteins in sufficient quantities for rigorous experimental analysis. By characterizing these proteins, scientists hope to identify novel targets for diagnostics and vaccines that could effectively boost the immune response against Kinetoplastida species. Moreover, understanding the biochemical pathways and surface presentations of KSDD proteins may aid in the design of inhibitors that disrupt parasite function. Therefore, ongoing research into KSDD recombinant proteins is not only relevant for basic biological understanding but also pivotal for developing innovative strategies to combat Kinetoplastida-related diseases, which pose significant public health challenges, particularly in endemic regions of Africa and South America.
