Analytical Data
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Gene name
FGFR-1
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简介
FGFR-1 protein is a tyrosine-protein kinase that acts as a cell surface receptor for fibroblast growth factor and plays a critical role in the complex regulation of embryonic development, cell proliferation, differentiation, and migration. FGFR-1 mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1, MAP kinase signaling pathways, and AKT1 signaling pathways. FGFR-1 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived FGFR-1 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Biological Activity
Measured by its ability to inhibit FGF-acidic dependent proliferation of Balb/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.3610ng/mL, corresponding to a specific activity is 2.770×106 units/mg. Measured by its ability to inhibit FGF-acidic dependent proliferation of Balb/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.3610ng/mL, corresponding to a specific activity is 2.770×106 units/mg.
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Alternative Names
Fibroblast growth factor receptor 1; FGFR-1; BFGFR; FLT-2; CD331; HBGFR
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Species
Rhesus Macaque
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
H9FRD4
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Expression Region
R22-Y283
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AA Sequence
RPSPTLPEQDALPSSEDDDDDDDSSSEEKETDNTKPNPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGVNTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGLSHHSAWLTVLEALEERPAVMTSPLY
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Protein Length
Partial
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Molecular Weight
50-75 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Fibroblast growth factor receptor 1 (FGFR-1) is a crucial receptor that plays vital roles in various biological processes, including cellular proliferation, differentiation, and survival. Research on FGFR-1 has gained momentum due to its significant implications in developmental biology and pathophysiology, particularly in cancer. Abnormal FGFR-1 signaling has been linked to various malignancies, including breast, lung, and bladder cancers, making it a target for therapeutic intervention. The development of recombinant FGFR-1 proteins has been instrumental in elucidating its structural and functional properties. By generating these proteins through recombinant DNA technology, researchers can produce pure and functional FGFR-1 to study its interactions with ligands, downstream signaling pathways, and the effects of mutations associated with cancer. Additionally, understanding the molecular mechanisms governing FGFR-1 activity can aid in the design of FGFR-targeted therapies, such as selective inhibitors and antibodies, offering potential treatment strategies for FGFR-1-driven tumors. The advancement of FGFR-1 recombinant protein studies has also provided insights into the receptor's role in tissue regeneration and repair, highlighting its importance beyond oncology. As research continues to uncover the complexities of FGFR-1 signaling, it remains a key focus in both basic and translational research aimed at improving disease outcomes through targeted molecular therapies.
