Analytical Data
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Gene name
PTCH2
- Application
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Species
Human
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y6C5
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Expression Region
79-392aa
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Molecular Weight
40.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PTCH2, or Patched2, is a key component of the Hedgehog signaling pathway, which plays a crucial role in regulating embryonic development and tissue homeostasis. This pathway is essential for cellular communication during development, influencing processes such as cell proliferation, differentiation, and migration. PTCH2, being a complementary receptor to PTCH1, has gained interest in recent years due to its involvement in various biological processes and potential implications in cancer and developmental disorders. Research has indicated that mutations or aberrations in PTCH2 can be linked to tumorigenesis, particularly in basal cell carcinoma and medulloblastoma. Moreover, PTCH2 has been recognized for its regulatory effects on cholesterol metabolism, further highlighting its significance in both developmental biology and disease states. The production of recombinant PTCH2 protein has become a focus of research to facilitate the understanding of its structure-function relationships and interactions within the Hedgehog signaling pathway. By studying the recombinant protein, researchers aim to elucidate its role in cellular signaling and identify potential therapeutic targets for diseases associated with Hedgehog pathway dysregulation. This research holds promise for advancing our knowledge of cancer biology and developing targeted therapies that could improve patient outcomes in Hedgehog-associated tumors.
