Analytical Data
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Gene name
DNAJA1
- Application
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Alternative Names
DnaJ protein homolog 2 (HSDJ) (Heat shock 40 kDa protein 4) (Heat shock protein J2) (HSJ-2) (Human DnaJ protein 2) (hDj-2)
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Species
Human
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P31689
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Expression Region
1-394aa
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Molecular Weight
52.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DNAJA1, a member of the Hsp40 family of heat shock proteins, plays a crucial role in protein folding, maintaining cellular proteostasis, and mediating responses to stress. Its functional significance has garnered attention in various biological and medical research domains. Recent studies have illustrated that DNAJA1 not only assists in the proper folding and maturation of newly synthesized proteins but also aids in the degradation of misfolded proteins through the proteasomal and autophagic pathways. Given its implications in neurodegenerative diseases, cancer, and metabolic disorders, researchers have been investigating DNAJA1 as a potential biomarker and therapeutic target. The recombinant expression of DNAJA1 allows for the detailed study of its interactions with client proteins and other molecular chaperones, thereby elucidating its biological mechanisms. Additionally, recombinant DNAJA1 can be employed in high-throughput screening assays to identify small molecules that can modulate its activity, offering promising avenues for drug discovery. Understanding the nuances of DNAJA1 function and regulation not only deepens our insight into cellular stress responses but also paves the way for innovative therapeutic strategies aimed at diseases linked to protein misfolding and aggregation.
