Analytical Data
-
Gene name
PMT3
- Application
-
Alternative Names
PMT3; YOR321W; O6148; Dolichyl-phosphate-mannose--protein mannosyltransferase 3; EC 2.4.1.109
-
Species
Saccharomyces cerevisiae
-
Source
E. coli
-
Tag
N- His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P47190
-
Expression Region
304-602aa
-
Molecular Weight
39.6 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PMT3 (Protein Methyl Transferase 3) is a significant enzyme involved in the post-translational modification of proteins through methylation, a process that plays a crucial role in regulating protein function, stability, and interactions. This enzyme, primarily studied in the context of various organisms including yeast and higher eukaryotes, catalyzes the transfer of methyl groups to lysine residues on target proteins, thereby influencing numerous cellular processes such as signal transduction, gene expression, and cellular differentiation. The research surrounding PMT3 has gained momentum due to its implications in various diseases, including cancer and neurodegenerative disorders, where abnormal protein methylation patterns are often observed. Understanding the structure and function of PMT3, as well as its role in cellular pathways, has become paramount for developing therapeutic strategies that could target aberrant methylation. Furthermore, the potential to utilize PMT3 as a biotechnological tool in protein engineering and synthetic biology adds another layer of interest, driving research to explore the enzyme's mechanism and its application in producing modified proteins with enhanced or novel functionalities. Overall, the study of PMT3 continues to be a vibrant field that bridges basic biology, disease research, and applied science.
