Analytical Data
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Gene name
ctfA
- Application
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Alternative Names
Acetoacetyl-CoA:acetate/butyrate:CoA transferase subunit A (Coat A)
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Species
Clostridium acetobutylicum
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P33752
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Expression Region
1-218aa
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Molecular Weight
30.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The research on CTF-A recombinant protein is situated at the intersection of molecular biology and biotechnology, with significant implications in understanding cellular processes and developing therapeutic applications. CTF-A, or Chromatin-Dependent Transcription Factor A, plays a crucial role in regulating gene expression through its interaction with chromatin and transcription machinery. Its function is vital in various biological processes, including cell differentiation and response to stress. Given its importance, scientists have focused on generating recombinant forms of CTF-A to study its structure-function relationships and to elucidate its mechanisms of action at a molecular level. The production of CTF-A as a recombinant protein allows for in-depth analysis of its biochemical properties and interactions with other cellular components. This research is particularly relevant in the context of diseases where transcriptional regulation is disrupted, such as cancer and genetic disorders. By harnessing recombinant DNA technology, researchers can manipulate the CTF-A protein for various experimental designs, aiding in the development of targeted therapies and diagnostic tools. Furthermore, understanding CTF-A's role can provide insights into epigenetic regulation and its implications in developmental biology and disease progression, thus underscoring the significance of this research in advancing both basic science and clinical applications.
