Analytical Data
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Gene name
ORF1
- Application
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Alternative Names
ORF1; Non-structural polyprotein pORF1 [Includes: Methyltransferase; EC 2.1.1.-; EC 2.7.7.-); Putative papain-like cysteine protease; PLP; EC 3.4.22.-); NTPase/helicase; EC 3.6.4.-); RNA-directed RNA polymerase; RdRp; EC 2.7.7.48)]
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Species
Hepatitis E virus genotype 1
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P33424
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Expression Region
60-240aa
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Molecular Weight
24.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The ORF1 recombinant protein, derived from the open reading frame 1 (ORF1) of various viruses, has garnered attention in the field of virology and molecular biology due to its role in viral replication and pathogenesis. ORF1 typically encodes essential non-structural proteins that are crucial for the viral life cycle, including those involved in replication, transcription, and modulation of host immune responses. Understanding the structure and function of ORF1 proteins can provide insights into viral mechanisms and facilitate the development of therapeutic strategies. Research has shown that these proteins can exhibit significant variability among different viral strains, suggesting an evolutionary adaptation to diverse host environments. As recombinant DNA technology has advanced, scientists have been able to produce ORF1 proteins in vitro for functional studies, thus aiding in vaccine development and antiviral drug design. These studies are vital for identifying potential targets for intervention and for elucidating the intricate interplay between viruses and their hosts. In particular, given the ongoing emergence of novel viruses, understanding ORF1 and its protein products is essential for preemptive measures and public health preparedness.
