Analytical Data
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基因名
Autolysin
- Application
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别名
Gamete lytic enzyme
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种属
Chlamydomonas reinhardtii
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表达系统
E. coli
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标签
N- His-SUMO
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P31178
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表达区间
184-638aa
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分子量
65.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Autolysins are enzymes that play a crucial role in bacterial cell wall remodeling, particularly during growth and division, by breaking down peptidoglycan components. They are important for maintaining the structural integrity of the bacterial cell wall while enabling cell growth and division. The study of autolysins has garnered significant interest due to their potential applications in drug development and biotechnology. As antibiotic resistance becomes a pressing global health issue, autolysins represent a promising avenue for developing novel antimicrobial agents, especially against gram-positive bacteria. Recombinant autolysins, produced through genetic engineering techniques, allow for the study of their function, structure, and interactions at a molecular level. These studies can further elucidate the mechanisms of cell wall hydrolysis and may lead to the discovery of new therapeutic strategies. Additionally, understanding the regulation of autolysin activity can provide insights into bacterial physiology and pathogenicity. The research on recombinant autolysins not only enhances our fundamental understanding of bacterial biology but also offers a potential pathway for innovative approaches to combat bacterial infections in an era of increasing antibiotic resistance.
