Analytical Data
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Gene name
Autolysin
- Application
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Alternative Names
Gamete lytic enzyme
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Species
Chlamydomonas reinhardtii
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Source
E. coli
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Tag
N- His-SUMO
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P31178
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Expression Region
184-638aa
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Molecular Weight
65.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Autolysins are enzymes that play a crucial role in bacterial cell wall remodeling, particularly during growth and division, by breaking down peptidoglycan components. They are important for maintaining the structural integrity of the bacterial cell wall while enabling cell growth and division. The study of autolysins has garnered significant interest due to their potential applications in drug development and biotechnology. As antibiotic resistance becomes a pressing global health issue, autolysins represent a promising avenue for developing novel antimicrobial agents, especially against gram-positive bacteria. Recombinant autolysins, produced through genetic engineering techniques, allow for the study of their function, structure, and interactions at a molecular level. These studies can further elucidate the mechanisms of cell wall hydrolysis and may lead to the discovery of new therapeutic strategies. Additionally, understanding the regulation of autolysin activity can provide insights into bacterial physiology and pathogenicity. The research on recombinant autolysins not only enhances our fundamental understanding of bacterial biology but also offers a potential pathway for innovative approaches to combat bacterial infections in an era of increasing antibiotic resistance.
