Analytical Data
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Gene name
3-hydroxybutyrate
- Application
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Alternative Names
Poly(3-hydroxybutyrate) depolymerase; PHB depolymerase; EC 3.1.1.75
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Species
Ralstonia pickettii
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P12625
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Expression Region
28-488aa
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Molecular Weight
50.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
3-Hydroxybutyrate, a key metabolite in the ketone body synthesis pathway, plays a vital role in energy metabolism, particularly during periods of fasting, prolonged exercise, or carbohydrate restriction. The significance of 3-hydroxybutyrate extends beyond mere energy provision; it also exhibits neuroprotective effects and has potential therapeutic implications for various conditions, including diabetes, neurological disorders, and obesity. Recent studies have focused on the production and characterization of recombinant proteins containing 3-hydroxybutyrate to better understand its biological functions and mechanisms of action. These recombinant proteins can facilitate the investigation of enzyme pathways involved in ketogenesis and the integration of 3-hydroxybutyrate into metabolic networks. By utilizing advanced techniques such as genetic engineering and synthetic biology, researchers aim to optimize the yield and function of these proteins, paving the way for novel applications in biotechnology and medicine. Furthermore, the exploration of 3-hydroxybutyrate's role in cellular signaling and its influence on gene expression highlights its potential as a biomarker and therapeutic target. Thus, the study of 3-hydroxybutyrate-related recombinant proteins emerges as a promising frontier in metabolic research, offering insights into metabolic regulation and novel avenues for treatment strategies in metabolic disorders.
