Analytical Data
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基因名
TLR3/CD283
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简介
The TLR3 protein is a key Toll-like receptor family member that plays an important role in the innate immune system's recognition of pathogen-associated molecular patterns, especially viral double-stranded RNA. Its research enhances understanding of immune responses and provides potential applications for vaccine development and antiviral treatments. TLR3 Protein, Human (HEK293, His) is the recombinant human-derived TLR3 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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生物活性
1.Immobilized Human TLR3 at 0.5 μg/mL (100μL/Well) on the plate. Dose response curve for Anti-TLR3 Antibody with the EC50 < 13.6 ng/mL determined by ELISA. 2.Measured by its ability to inhibit poly I:C induced IL-8 secretion by HEK293 human embryonic kidney cells transfected with TLR3. The ED50 for this effect is 4.109 µg/mL, corresponding to a specific activity is 243.368 U/mg in the presence of 0.2 µg/mL of poly I:C. Measured by its ability to inhibit poly I:C induced IL-8 secretion by HEK293 human embryonic kidney cells transfected with TLR3. The ED50 for this effect is 4.109 µg/mL, corresponding to a specific activity is 243.368 U/mg in the presence of 0.2 µg/mL of poly I:C.
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别名
TLR3; CD283; IIAE2
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种属
Human
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表达系统
HEK293
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标签
C-His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
Q6PCD4
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表达区间
S23-E703
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氨基酸序列
SSTTKCTVSHEVADCSHLKLTQVPDDLPTNITVLNLTHNQLRRLPAANFTRYSQLTSLDVGFNTISKLEPELCQKLPMLKVLNLQHNELSQLSDKTFAFCTNLTELHLMSNSIQKIKNNPFVKQKNLITLDLSHNGLSSTKLGTQVQLENLQELLLSNNKIQALKSEELDIFANSSLKKLELSSNQIKEFSPGCFHAIGRLFGLFLNNVQLGPSLTEKLCLELANTSIRNLSLSNSQLSTTSNTTFLGLKWTNLTMLDLSYNNLNVVGNDSFAWLPQLEYFFLEYNNIQHLFSHSLHGLFNVRYLNLKRSFTKQSISLASLPKIDDFSFQWLKCLEHLNMEDNDIPGIKSNMFTGLINLKYLSLSNSFTSLRTLTNETFVSLAHSPLHILNLTKNKISKIESDAFSWLGHLEVLDLGLNEIGQELTGQEWRGLENIFEIYLSYNKYLQLTRNSFALVPSLQRLMLRRVALKNVDSSPSPFQPLRNLTILDLSNNNIANINDDMLEGLEKLEILDLQHNNLARLWKHANPGGPIYFLKGLSHLHILNLESNGFDEIPVEVFKDLFELKIIDLGLNNLNTLPASVFNNQVSLKSLNLQKNLITSVEKKVFGPAFRNLTELDMRFNPFDCTCESIAWFVNWINETHTNIPELSSHYLCNTPPHYHGFPVRLFDTSSCKDSAPFE
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蛋白长度
Partial
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分子量
90-130 kDa.
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TLR3, or Toll-like receptor 3, is a vital component of the innate immune system, playing a crucial role in the recognition of viral infections and the subsequent activation of immune responses. It specifically identifies double-stranded RNA, a common byproduct of viral replication, which triggers signaling pathways that lead to the production of pro-inflammatory cytokines and type I interferons. The exploration of TLR3/CD283 recombinant protein is significant for understanding its molecular mechanisms and potential therapeutic applications, particularly in antiviral therapies and vaccine development. The ability to produce recombinant TLR3 enhances research on its structure-function relationships and facilitates the study of its interactions with various ligands. Additionally, TLR3 has been implicated in various diseases, including autoimmune disorders and cancers, making it an attractive target for drug development. By investigating the properties and effects of TLR3/CD283 recombinant protein, researchers aim to elucidate its role in immune regulation and identify novel strategies to manipulate TLR3 pathways for therapeutic benefits. This research can ultimately contribute to the design of immunomodulatory treatments that enhance the immune response against pathogens or mitigate undesirable inflammatory reactions.
