Analytical Data
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Gene name
rbsK
- Application
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Alternative Names
rbsK; b3752; JW3731; Ribokinase; RK; EC 2.7.1.15
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Species
Escherichia coli
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0A9J6
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Expression Region
1-309aa
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Molecular Weight
36.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RbsK, or ribose kinase, is an enzyme that plays a crucial role in the metabolism of ribose, a sugar that is a key component of nucleotides and nucleic acids. Its primary function is to catalyze the phosphorylation of ribose to ribose-5-phosphate, a critical step in the pentose phosphate pathway, which is essential for nucleotide synthesis and cellular metabolism. Understanding the structure and function of RbsK is important for several reasons. Firstly, it can provide insights into the regulatory mechanisms of ribose metabolism, which is vital for cellular growth and division. Secondly, RbsK has potential implications in biotechnology and medicine, as manipulating its activity could lead to advances in metabolic engineering and the development of novel therapeutic strategies. Given the rising interest in metabolic pathways for bioengineering purposes, researchers are increasingly focused on characterizing RbsK, determining its kinetics, and elucidating its structure through recombinant protein production. This approach enables a better understanding of enzyme function and lays the groundwork for potential applications in synthesizing bio-based products or enhancing microbial cell factories. Furthermore, examining RbsK in various organisms can reveal evolutionary adaptations and variations in metabolic pathways, contributing to our knowledge of metabolic diversity across different life forms. Overall, RbsK represents a significant target for research in enzymology and metabolic engineering, with promising applications in health and industry.
