Analytical Data
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基因名
GTF2F2
- Application
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别名
ATP-dependent helicase GTF2F2General transcription factor IIF 30KDA subunitTranscription initiation factor IIF subunit beta ;TFIIF-betaTranscription initiation factor RAP30
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种属
Human
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表达系统
E. coli
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标签
N- His-SUMO
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P13984
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表达区间
2-249aa
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分子量
44.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GTF2F2, or General Transcription Factor IIH, is a pivotal component involved in the transcription initiation process within eukaryotic cells. This multi-subunit complex plays a crucial role in unwinding DNA, enabling RNA polymerase II to access the gene coding regions for transcription. Research into GTF2F2's recombinant protein is essential due to its implications in various cellular processes, including DNA repair, transcription regulation, and cell cycle control. Mutations or dysfunctions in the components of this complex have been linked to several pathologies, including cancer and genetic disorders. Understanding GTF2F2 at the protein level allows scientists to dissect its structural and functional characteristics, revealing insights into its mechanistic roles in gene expression and cellular homeostasis. Moreover, recombinant GTF2F2 proteins serve as valuable tools for studying transcriptional regulation and for developing potential therapeutic strategies targeting associated diseases. The production and analysis of GTF2F2 in a laboratory setting facilitate a deeper understanding of its interactions with other transcription factors and the broader regulatory networks that govern gene expression, ultimately contributing to advancements in molecular biology and medicine.
