Analytical Data
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Gene name
CDC25C
- Application
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Alternative Names
Dual specificity phosphatase Cdc25C
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Species
Human
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P30307
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Expression Region
1-473aa
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Molecular Weight
57.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CDC25C is a crucial member of the CDC25 phosphatase family, known for its role in regulating the cell cycle by activating cyclin-dependent kinases (CDKs). This protein is particularly important during the G2/M transition of the cell cycle, where it dephosphorylates and activates CDK1, facilitating mitotic entry. Dysregulation of CDC25C has been implicated in various cancers, making it a significant target for therapeutic interventions. Research on recombinant CDC25C protein aims to elucidate its structural and functional properties, providing insights into its enzymatic activity and regulatory mechanisms. By producing and characterizing this protein in vitro, scientists can study its interaction with other cell cycle regulators, investigate its role in tumorigenesis, and potentially explore its utility as a biomarker or therapeutic target in cancer treatment. Understanding the dynamics of CDC25C not only enhances our comprehension of cell cycle control but also opens avenues for developing novel cancer therapies that can selectively target proliferating cells with aberrant CDC25C activity.
