Analytical Data
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Gene name
CAPZB
- Application
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Alternative Names
CapZ beta
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Species
Human
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Source
E. coli
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Tag
N- GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P47756
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Expression Region
1-272aa
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Molecular Weight
57.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CAPZB, the beta subunit of the heteromeric CapZ protein complex, plays a crucial role in the regulation of actin dynamics by capping the barbed ends of actin filaments, thus preventing further polymerization. Research on CAPZB and its interactions is significant for understanding various cellular processes, including cell motility, shape maintenance, and cytokinesis, as well as its implications in diseases such as cancer, where aberrant actin dynamics contribute to metastatic behavior. The study of recombinant CAPZB is particularly important for elucidating its structural and functional properties, facilitating insights into its role in actin remodeling. Furthermore, recombinant CAPZB can serve as a valuable tool in biochemical assays and structural biology studies, enabling researchers to dissect the mechanistic details of actin filament regulation and explore potential therapeutic targets for conditions linked to actin pathology. Advancements in recombinant protein expression and purification techniques have opened new avenues for producing high-quality CAPZB, allowing for comprehensive studies into its biophysical characteristics, binding affinities, and interaction networks within the cellular environment. Overall, the investigation of CAPZB serves as a pivotal component in the broader context of cytoskeletal research, contributing to our understanding of cellular architecture and function.
