Analytical Data
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Gene name
Blm
- Application
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Alternative Names
RecQ helicase homolog
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Species
Mouse
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Source
E. coli
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Tag
N- His & C- MYC
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O88700
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Expression Region
684-859aa
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Molecular Weight
24.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
BLM (Bloom syndrome protein) is a crucial component of the homologous recombination repair pathway, responsible for maintaining genomic stability. Research on BLM has gained significance due to its association with Bloom syndrome, a rare genetic disorder characterized by a heightened predisposition to cancer and other age-related diseases. The BLM protein acts as a helicase, unwinding DNA during replication and repair processes, which is essential for accurate chromosome segregation. Deficiencies or mutations in the BLM gene can lead to increased genomic instability, contributing to cancer development. Understanding the structure, function, and regulation of BLM is vital for elucidating its role in maintaining genomic integrity and its implications in cancer biology. Recent advances in molecular biology and genetic engineering have provided insights into the biochemical properties of BLM and its interactions with other proteins involved in DNA repair. Research in this area not only enhances our understanding of Bloom syndrome but also opens avenues for targeted therapeutic strategies aimed at cancers associated with defective DNA repair mechanisms.
